Coding

Part:BBa_K2929002:Design

Designed by: James Hammond   Group: iGEM19_St_Andrews   (2019-10-10)


Human IgE CH3 Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 334
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added 5' NcoI and 3' BamHI cut sites to allow cloning into our vector.


Source

CH3 Domain isolated using the crystal structure on the PDB (PDB ID: 5MOL). CH3 Domain was identified in Pymol and then the CH2 Domain deleted from the protein sequence. DNA sequence obtained by reverse translation of the protein sequence.


References